{"openapi":"3.1.0","info":{"title":"PubMed API","version":"1.0.0","description":"Search the world’s biomedical literature and retrieve clean article metadata and abstracts, powered by the U.S. National Library of Medicine’s PubMed via NCBI E-utilities. Search 35+ million citations by keyword with relevance or date sorting, look up one or more articles by PubMed id for title, authors, journal, publication date, volume/issue/pages, DOI and publication type, or fetch the full abstract text for a paper. Authoritative open data returned as tidy JSON through a fast, reliable API. 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CRISPR-Cas system is very efficient, fast, easy and cheap technique for achieving knock-out gene in the cell. CRISPR-Cas9 system refurbishes the targeted genome editing approach into a more expedient and competent way, thus facilitating proficient genome editing through embattled double-strand breaks in approximately any organism and cell type. The off-target effects of CRISPR Cas system has been circumnavigated by using paired nickases. Moreover, CRISPR-Cas9 has been used effectively for numerous purposes, like knock-out of a gene, regulation of endogenous gene expression, live-cell labelling of chromosomal loci, edition of single-stranded RNA and high-throughput gene screening. The execution of the CRISPR-Cas9 system has amplified the number of accessible scientific substitutes for studying gene function, thus enabling generation of CRISPR-based disease models. Even though many mechanistic questions are left behind to be answered and the system is not yet fool-proof i.e., a number of challenges are yet to be addressed, the employment of CRISPR-Cas9-based genome engineering technologies will increase our understanding to disease processes and their treatment in the near future. In this review we have discussed the history of CRISPR-Cas9, its mechanism for genome editing and its application in animal, plant and protozoan parasites. Additionally, the pros and cons of CRISPR-Cas9 and its potential in therapeutic application have also been detailed here.","language":"eng","pub_date":"2019 Sep 1","epub_date":"2019 Jul 8","pub_types":["Journal Article","Review"]},"meta":{"timestamp":"2026-05-30T18:16:52.545Z","request_id":"28f3a271-c9bc-43a3-8131-9def61908eda"},"status":"ok","message":"Article retrieved","success":true}}}},"401":{"description":"Missing or invalid x-oanor-key header"},"402":{"description":"Active subscription required"},"429":{"description":"Rate-limit or monthly quota reached"},"502":{"description":"Upstream did not respond"}}}},"/v1/search":{"get":{"operationId":"get_v1_search","tags":["PubMed"],"summary":"Search PubMed","description":"","parameters":[{"name":"query","in":"query","required":true,"description":"Search terms (PubMed syntax supported)","schema":{"type":"string"},"example":"CRISPR gene editing"},{"name":"limit","in":"query","required":false,"description":"1-50 (default 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