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API · /dnamelt-api

DNA Melting Temperature API

healthy 3,544 Subscribers

DNA-oligo and PCR-primer maths as an API, computed locally and deterministically. The tm endpoint computes the melting temperature of a primer sequence three ways: the Wallace rule 2·(A+T) + 4·(G+C) for short oligos up to 13 nt, the Marmur–Wallace GC formula 64.9 + 41·(nGC − 16.4)/N for longer ones, and the salt-adjusted 81.5 + 0.41·%GC − 675/N + 16.6·log10[Na+] for a given sodium concentration, and recommends the right method for the length — an eight-base ATGCATGC melts at 24 °C by Wallace, a 20-base 50 %-GC primer at about 51.8 °C by Marmur. The gc-content endpoint reports the GC and AT percentages, the per-base counts and the single-stranded molecular weight. The reverse-complement endpoint returns the complement, the reverse and the reverse complement of a strand. Sequences use A/C/G/T (case-insensitive, whitespace ignored) and [Na+] is in mol/L. Everything is computed locally and deterministically, so it is instant and private. Ideal for molecular-biology, biotech, PCR, primer-design, bioinformatics and lab-automation app developers, oligo and primer calculators, and LIMS software. Estimation formulas for primer design, not a substitute for nearest-neighbour thermodynamics. Pure local computation — no key, no third-party service, instant. Live, nothing stored. 3 endpoints. This is oligo melting temperature; for population-genetics allele frequencies use a genetics API.

#dna #pcr-primer #melting-temperature #gc-content #bioinformatics #developer-tools
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/api/dnamelt-api/openapi.json
/api/dnamelt-api/llms.txt

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DNA Melting Temperature API — live data on the oanor API marketplace

API health

healthy
Uptime
100.00%
Server probes · 24h
Avg latency
77 ms
Server probes · 24h
Subscribers
3,544
active
Total calls
76
last 7 days
status Full status page → · 8 probes/24h

Pricing

Pick a tier — billed monthly, cancel anytime.

Free

Free

  • 3,850 calls / month
  • 2 requests / second
  • Hard cap (429 above quota, no overage)
  • 3,850 calls/month
  • 2 req/sec
  • Tm (3 methods) + GC + reverse complement
  • No credit card
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Starter

€6.40 /month

  • 36,500 calls / month
  • 6 requests / second
  • Hard cap (429 above quota, no overage)
  • 36,500 calls/month
  • 6 req/sec
  • Salt-adjusted Tm, molecular weight
  • Email support
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Pro

€18.50 /month

  • 174,000 calls / month
  • 15 requests / second
  • Hard cap (429 above quota, no overage)
  • 174,000 calls/month
  • 15 req/sec
  • Primer-design & LIMS pipelines
  • Priority support
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Mega

€57.00 /month

  • 1,045,000 calls / month
  • 40 requests / second
  • Hard cap (429 above quota, no overage)
  • 1,045,000 calls/month
  • 40 req/sec
  • Platform scale
  • Dedicated SLA
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DNA/RNA sequence-analysis maths as an API, computed locally and deterministically. The analyze endpoint reports the length and base composition of a sequence, the GC and AT content, the complement, the reverse and the reverse complement (the opposite strand read 5'→3'), and the approximate single-stranded molecular weight. The translate endpoint transcribes DNA to mRNA (T→U) and translates it to protein with the standard genetic code in reading frame 1, 2 or 3, giving the one-letter amino-acid sequence, the protein length and the number of stop codons. The melting endpoint estimates a primer's melting temperature with the Wallace rule, 4·(G+C) + 2·(A+T), for short oligos and a salt-adjusted basic formula for longer ones. Sequences are case- and whitespace-insensitive and accept A, C, G, T for DNA or U for RNA. Everything is computed locally and deterministically, so it is instant and private. Ideal for bioinformatics, molecular-biology, genomics and lab app developers, primer-design and sequence-inspection tools, and biology education. Pure local computation — no key, no third-party service, instant. Live, nothing stored. 3 endpoints. This is sequence analysis; for genome assembly data use a genomes API.

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Frequently asked questions

Quick answers about pricing, quotas, and integration.

How do I get an API key for DNA Melting Temperature API?
Sign up for free at oanor.com, generate an API key from the developer dashboard, and call DNA Melting Temperature API with the x-oanor-key header. No credit card needed for the free tier.
What's the rate limit for DNA Melting Temperature API?
Free tier allows 1 request per second. Paid plans scale up to 50 requests per second on the Mega tier. Hard limits return HTTP 429 above the quota — no surprise overage charges.
How much does DNA Melting Temperature API cost?
DNA Melting Temperature API has a free tier with 100 calls / month. Paid plans start at €6.40 / month with higher quotas and faster rate limits.
Can I cancel my subscription anytime?
Yes. Plans are billed monthly and you can cancel anytime from your billing dashboard. No long-term contracts and no cancellation fee.
Is DNA Melting Temperature API GDPR-compliant?
All requests to DNA Melting Temperature API go through our EU-based gateway. Your upstream API key never leaves our server and no personal data is shared with the upstream provider beyond the request you send.

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curl https://api.oanor.com/dnamelt-api/SOME_PATH \
  -H "x-oanor-key: oanor_test_..."
const res = await fetch("https://api.oanor.com/dnamelt-api/SOME_PATH", {
  headers: { "x-oanor-key": "oanor_test_..." }
});
const data = await res.json();
$ch = curl_init("https://api.oanor.com/dnamelt-api/SOME_PATH");
curl_setopt($ch, CURLOPT_RETURNTRANSFER, true);
curl_setopt($ch, CURLOPT_HTTPHEADER, ["x-oanor-key: oanor_test_..."]);
$response = curl_exec($ch);
import requests
r = requests.get(
    "https://api.oanor.com/dnamelt-api/SOME_PATH",
    headers={"x-oanor-key": "oanor_test_..."},
)
print(r.json())

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